Ku, the heterodimer of Ku70 and Ku80, plays an essential role in the DNA double-strand break (DSB) repair pathway, i.e., non-homologous end-joining (NHEJ). Two isoforms of Ku80 encoded by the same genes, namely, Ku80 and KARP-1 are expressed and function in primate cells, but not in rodent cells.

The propensity for Ku70 to associate with Ku80 and to bind DNA correlates with the ability to activate DNA-PK, although two mutants showed that the roles of Ku70 in DNA-PK activation and IR repair

2011-01-12 The proteins Ku70 (69.8 kDa) and Ku80 (82.7 kDa) form a heterodimeric complex that is an essential component of the nonhomologous end joining DNA double-strand break repair pathway in mammalian cells. Interaction of Ku with DNA is central for the functions of Ku. 2001-03-01 Ku70‐knockout mice, in contrast, appear to be tumor prone and develop thymic lymphomas with high incidence (30, 36). Finally, in agreement with a Ku70‐specific role in life‐span regulation, it was recently shown that knockdown of Ku70, but not Ku80, significantly extended life span in the p53‐null background . 2020-10-27 Ku80 is a protein that, in humans, is encoded by the XRCC5 gene.

Ku70 and ku80 function

The information may be useful in examining the function of the Ku complex, as well Pull-down analysis with cytosolic proteins identified Ku70 and Ku80 as the The central domain of Ku70 and Ku80 is a DNA-binding beta-barrel domain. Ku makes only a few contacts with the sugar-phosphate backbone, and none with the 1 Oct 2018 Key Words: Ku70SHP-1SIRT1DNA damage repairNon-homologous end joining or DNA-targeting heterodimer (KU70 and KU80) are more sensitive to DNA To investigate the role of KU70 in response to DNA damage, we 6 Oct 2020 Knockdown of Ku70 or Ku80 in naïve mice elicited mitochondrial collapse or ER stress, leading to bronchial epithelial cell apoptosis and Together, Ku70 and Ku80 promote LPS-induced NFκB activation and break repair by Ku70 requires heterodimerization with Ku80 and DNA binding functions . 29 Aug 2018 For instance, cytoplasmic Ku70 independent of Ku80 suppresses the dimerization of Ku70 and Ku80 and to the nuclear transport function, Thus, the Ku protein is involved in DNA repair and in V(D)J recombination, and these results may also indicate a role for the Ku-DNA-dependent protein kinase suggest that the binding of the Ku80 and Ku70 proteins to DNA plays an important role in the joining of both signal and coding ends. On the other hand, the scid previous reports that show Ku70 and Ku80 have functions independent of the Ku heterodimer [17]. Results. Previous reports suggest that ku80J/J mutant mice 21 Feb 2003 Arabidopsis Ku70 and Ku80 proteins form a heterodimer with DNA binding The essential role of the Ku70/Ku80 heterodimer in telomere 26 Nov 2014 eukaryotes, Ku is a heterodimer comprised of two subunits, Ku70 and Ku80, that is best characterized for its central role as the initial DNA end 24 Jul 2015 Ku80 and ku70 form the heterodimer complex Ku, required for proper Organism, UniProt Accession, Gene, Functional Information.

function of Ku70 at DSB sites might play a crucial role in modulating the NHEJ repair pathway [3, 14, 20, 26, 28]. There is, however, no report describing the localization and function of canine Ku70. In this study, we have cloned Ku70 cDNA from a beagle dog testis library and conducted comparative analysis to understand the

2 Genetik. Der Ku70/Ku80-Komplex wird durch zwei Gene codiert: XRCC6 und XRCC5.

Ku80 is best known for its function in DNA damage repair in its heterodimer with Ku70 [20], while accumulating evidence has suggested that Ku80 also plays important roles in cellular processes

Der Ku70/Ku80-Komplex wird durch zwei Gene codiert: XRCC6 und XRCC5.

Results. Previous reports suggest that ku80J/J mutant mice 21 Feb 2003 Arabidopsis Ku70 and Ku80 proteins form a heterodimer with DNA binding The essential role of the Ku70/Ku80 heterodimer in telomere 26 Nov 2014 eukaryotes, Ku is a heterodimer comprised of two subunits, Ku70 and Ku80, that is best characterized for its central role as the initial DNA end 24 Jul 2015 Ku80 and ku70 form the heterodimer complex Ku, required for proper Organism, UniProt Accession, Gene, Functional Information. So basically edge server plays a role to make the computational decisions ahead of time, sending only filtered data to the cloud, saving both time and money. 24 Jul 2018 These three pins are the Switch, Output A and Output B respectively.
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A, Ku70/80 was purified from insect cells coinfected with recombinant baculoviruses expressing human His-Ku70 and -Ku80. Ku70/80 was incubated with PARP-1 in the presence of sonicated DNA and NAD + (lanes 1 and 3) or buffer only (lanes 2 and 4).

In Complex: Ku70:Ku80 complex Macromolecular complex annotations are imported from the Complex Portal . These annotations have been derived from physical molecular interaction evidence extracted from the literature and cross-referenced in the entry, or by curator inference from information on homologs in closely related species or by inference from scientific background. First, either Ku70 or Ku80 functions outside the Ku heterodimer such that deletion of one is not identical to deletion of the other.
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26 Nov 2014 eukaryotes, Ku is a heterodimer comprised of two subunits, Ku70 and Ku80, that is best characterized for its central role as the initial DNA end

There is, however, no report describing the localization and function of canine Ku70. In this study, we have cloned Ku70 cDNA from a beagle dog testis library and conducted comparative analysis to understand the Ku70 is the 70 kDa subunit of the lupus Ku autoantigen. The lupus Ku autoantigen was identified in individuals with systemic lupus erythematosus (SLE) and related disorders. The Ku antigen is a heterodimer of Ku70 and Ku80.

Ku80 is a protein that, in humans, is encoded by the XRCC5 gene. Together, Ku70 and Ku80 make up the Ku heterodimer, which binds to DNA double-strand break ends and is required for the non-homologous end joining (NHEJ) pathway of DNA repair.

KU70 and KU80 as top hits. The mech-anism by which KU70/80 recognizes dsDNAendsaspartoftheDNA-PK complex in the nucleus is well under-stood. Wang et al. (2021) found that a substantial amount of KU70/80 is also expressed in the cytoplasm of T cells, where the complex will bind to DNA, recruit DNA-dependent protein kinase catalytic subunit (DNA One of the functions of the abundant heterodimeric nuclear protein, Ku (Ku70/Ku80), is its involvement in the initiation of DNA replication through its ability to bind to chromoso

Sequence and domain structure of the zebrafish Ku80 gene. The Ku protein, composed of Ku70 and Ku80 subunits, carries out the initial DNA end recognition step in NHEJ, and is well characterized biochemically and genetically in mammalian systems ( 42).